4V3B: The Structure Of Alpha2,3-sialyltransferase Variant 1 From Pasteurella Dagmatis In Complex With The Donor Product Cmp

Structure-guided active-site redesign of a family GT-80 beta-D-galactoside sialyltransferase (from Pasteurella dagmatis) to change enzyme regioselectivity from alpha-2,3 in the wild type to alpha-2,6 in a P7H-M117A double mutant is reported. Biochemical data for sialylation of lactose together with protein crystal structures demonstrate highly precise enzyme engineering.
PDB ID: 4V3BDownload
MMDB ID: 128445
PDB Deposition Date: 2014/10/17
Updated in MMDB: 2015/04
Experimental Method:
x-ray diffraction
Resolution: 2.18  Å
Source Organism:
Similar Structures:
Biological Unit for 4V3B: monomeric; determined by author and by software (PISA)
Molecular Components in 4V3B
Label Count Molecule
Protein (1 molecule)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB