4UJ3: Crystal Structure Of Human Rab11-rabin8-fip3

The small GTPase Rab11 and its effectors FIP3 and Rabin8 are essential to membrane-trafficking pathways required for cytokinesis and ciliogenesis. Although effector binding is generally assumed to be sequential and mutually exclusive, we show that Rab11 can simultaneously bind FIP3 and Rabin8. We determined crystal structures of human Rab11-GMPPNP-Rabin8 and Rab11-GMPPNP-FIP3-Rabin8. The structures reveal that the C-terminal domain of Rabin8 adopts a previously undescribed fold that interacts with Rab11 at an unusual effector-binding site neighboring the canonical FIP3-binding site. We show that Rab11-GMPPNP-FIP3-Rabin8 is more stable than Rab11-GMPPNP-Rabin8, owing to direct interaction between Rabin8 and FIP3 within the dual effector-bound complex. The data allow us to propose a model for how membrane-targeting complexes assemble at the trans-Golgi network and recycling endosomes, through multiple weak interactions that create high-avidity complexes.
PDB ID: 4UJ3Download
MMDB ID: 131644
PDB Deposition Date: 2015/4/8
Updated in MMDB: 2015/09
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Similar Structures:
Biological Unit for 4UJ3: trimeric; determined by author and by software (PQS)
Molecular Components in 4UJ3
Label Count Molecule
Proteins (3 molecules)
Ras-related Protein Rab-11a(Gene symbol: RAB11A)
Molecule annotation
Rab-3a-interacting Protein(Gene symbol: RAB3IP)
Molecule annotation
Rab11 Family-interacting Protein 3(Gene symbol: RAB11FIP3)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB