4UGJ: Structure Of Bacillus Subtilis Nitric Oxide Synthase In Complex With 3-(2-(6-amino-4-methylpyridin-2-yl)ethyl)-5-( Methyl(2-(methylamino)ethyl)amino)benzonitrile

Citation:
Abstract
Nitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Development of potent and selective bacterial NOS inhibitors is complicated by the high active site sequence and structural conservation shared with the mammalian NOS isoforms. To exploit bacterial NOS for the development of new therapeutics, recognition of alternative NOS surfaces and pharmacophores suitable for drug binding is required. Here, we report on a wide number of inhibitor-bound bacterial NOS crystal structures to identify several compounds that interact with surfaces unique to the bacterial NOS. Although binding studies indicate that these inhibitors weakly interact with the NOS active site, many of the inhibitors reported here provide a revised structural framework for the development of new antimicrobials that target bacterial NOS. In addition, mutagenesis studies reveal several key residues that unlock access to bacterial NOS surfaces that could provide the selectivity required to develop potent bacterial NOS inhibitors.
PDB ID: 4UGJDownload
MMDB ID: 130297
PDB Deposition Date: 2015/3/22
Updated in MMDB: 2015/07
Experimental Method:
x-ray diffraction
Resolution: 1.78  Å
Source Organism:
Similar Structures:
Biological Unit for 4UGJ: dimeric; determined by author and by software (PISA)
Molecular Components in 4UGJ
Label Count Molecule
Proteins (2 molecules)
2
Nitric Oxide Synthase Oxygenase(Gene symbol: nosA)
Molecule annotation
Chemicals (8 molecules)
1
2
2
2
3
2
4
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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