4U48: Crystal structure of Salmonella alpha-2-macroglobulin

Citation:
Abstract
Alpha-2-macroglobulins (A2Ms) are plasma proteins that trap and inhibit a broad range of proteases and are major components of the eukaryotic innate immune system. Surprisingly, A2M-like proteins were identified in pathogenically invasive bacteria and species that colonize higher eukaryotes. Bacterial A2Ms are located in the periplasm where they are believed to provide protection to the cell by trapping external proteases through a covalent interaction with an activated thioester. Here we report the crystal structures and characterization of Salmonella enterica ser. Typhimurium A2M in different states of thioester activation. The structures reveal thirteen domains whose arrangement displays high similarity to proteins involved in eukaryotic immune defence. A structural lock mechanism maintains the stability of the buried thioester, a requirement for its protease-trapping activity. These findings indicate that bacteria have developed a rudimentary innate immune system whose mechanism mimics that of eukaryotes.
PDB ID: 4U48Download
MMDB ID: 123285
PDB Deposition Date: 2014/7/23
Updated in MMDB: 2015/09
Experimental Method:
x-ray diffraction
Resolution: 2.95  Å
Source Organism:
Similar Structures:
Biological Unit for 4U48: monomeric; determined by author and by software (PISA)
Molecular Components in 4U48
Label Count Molecule
Protein (1 molecule)
1
Putative Inner Membrane Lipoprotein
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.