4TVY: Apo resorufin ligase

Chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherichia coli lipoic acid ligase. X-ray crystallography showed that the design closely matched the experimental structure. Resorufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular proteins genetically fused to a 13-aa recognition peptide in multiple mammalian cell lines and in primary cultured neurons. We used resorufin ligase to perform superresolution imaging of the intermediate filament protein vimentin by stimulated emission depletion and electron microscopies. This work illustrates the power of Rosetta for major redesign of enzyme specificity and introduces a tool for minimally invasive, highly specific imaging of cellular proteins by both conventional and superresolution microscopies.
PDB ID: 4TVYDownload
MMDB ID: 124112
PDB Deposition Date: 2014/6/28
Updated in MMDB: 2017/12
Experimental Method:
x-ray diffraction
Resolution: 2.151  Å
Source Organism:
Similar Structures:
Biological Unit for 4TVY: monomeric; determined by author
Molecular Components in 4TVY
Label Count Molecule
Protein (1 molecule)
Lipoate-protein Ligase a(Gene symbol: lplA)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB