National Center for
4TN8: Crystal structure of Thermus Thermophilus thioredoxin solved by sulfur SAD using Swiss Light Source data
Nat. Methods (2015) 12 p.131-133
We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.