4R4O: Crystal structure of RPA70N in complex with 5-(4-((4-(5-carboxyfuran-2-yl)benzyl)carbamothioyl)phenyl)-1-(3,4-dichlorophenyl)-1H-pyrazole-3-carboxylic acid

Replication Protein A is the primary eukaryotic ssDNA binding protein that has a central role in initiating the cellular response to DNA damage. RPA recruits multiple proteins to sites of DNA damage via the N-terminal domain of the 70 kDa subunit (RPA70N). Here we describe the optimization of a diphenylpyrazole carboxylic acid series of inhibitors of these RPA-protein interactions. We evaluated substituents on the aromatic rings as well as the type and geometry of the linkers used to combine fragments, ultimately leading to submicromolar inhibitors of RPA70N protein-protein interactions.
PDB ID: 4R4ODownload
MMDB ID: 124882
PDB Deposition Date: 2014/8/19
Updated in MMDB: 2014/11
Experimental Method:
x-ray diffraction
Resolution: 1.33  Å
Source Organism:
Similar Structures:
Biological Unit for 4R4O: monomeric; determined by author and by software (PISA)
Molecular Components in 4R4O
Label Count Molecule
Protein (1 molecule)
Replication Protein a 70 KDA DNA-binding Subunit(Gene symbol: RPA1)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB