4QX0: Cry3A Toxin structure obtained by Serial Femtosecond Crystallography from in vivo grown crystals isolated from Bacillus thuringiensis and data processed with the cctbx.xfel software suite

Citation:
Abstract
It has long been known that toxins produced by Bacillus thuringiensis (Bt) are stored in the bacterial cells in crystalline form. Here we describe the structure determination of the Cry3A toxin found naturally crystallized within Bt cells. When whole Bt cells were streamed into an X-ray free-electron laser beam we found that scattering from other cell components did not obscure diffraction from the crystals. The resolution limits of the best diffraction images collected from cells were the same as from isolated crystals. The integrity of the cells at the moment of diffraction is unclear; however, given the short time ( approximately 5 micros) between exiting the injector to intersecting with the X-ray beam, our result is a 2.9-A-resolution structure of a crystalline protein as it exists in a living cell. The study suggests that authentic in vivo diffraction studies can produce atomic-level structural information.
PDB ID: 4QX0Download
MMDB ID: 122401
PDB Deposition Date: 2014/7/17
Updated in MMDB: 2014/10
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Similar Structures:
Biological Unit for 4QX0: monomeric; determined by author and by software (PISA)
Molecular Components in 4QX0
Label Count Molecule
Protein (1 molecule)
1
Pesticidal Crystal Protein Cry3aa
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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