4QUU: Structure Of The Bromodomain Of Human Atpase Family Aaa Domain- Containing Protein 2 (atad2) Complexed With Histone H4-k(ac)5

Citation:
Abstract
Although the conserved AAA ATPase and bromodomain factor, ATAD2, has been described as a transcriptional co-activator upregulated in many cancers, its function remains poorly understood. Here, using a combination of ChIP-seq, ChIP-proteomics, and RNA-seq experiments in embryonic stem cells where Atad2 is normally highly expressed, we found that Atad2 is an abundant nucleosome-bound protein present on active genes, associated with chromatin remodelling, DNA replication, and DNA repair factors. A structural analysis of its bromodomain and subsequent investigations demonstrate that histone acetylation guides ATAD2 to chromatin, resulting in an overall increase of chromatin accessibility and histone dynamics, which is required for the proper activity of the highly expressed gene fraction of the genome. While in exponentially growing cells Atad2 appears dispensable for cell growth, in differentiating ES cells Atad2 becomes critical in sustaining specific gene expression programmes, controlling proliferation and differentiation. Altogether, this work defines Atad2 as a facilitator of general chromatin-templated activities such as transcription.
PDB ID: 4QUUDownload
MMDB ID: 121984
PDB Deposition Date: 2014/7/12
Updated in MMDB: 2014/08
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 4QUU: dimeric; determined by author and by software (PISA)
Molecular Components in 4QUU
Label Count Molecule
Proteins (2 molecules)
1
Atpase Family AAA Domain-containing Protein 2(Gene symbol: ATAD2)
Molecule annotation
1
Histone H4(Gene symbol: HIST1H4I)
Molecule annotation
Chemicals (10 molecules)
1
1
2
9
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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