4QTJ: Complex of WOPR domain of Wor1 in Candida albicans with the 13bp dsDNA

Wor1 (white-opaque switching regulator 1) is a master regulator of the white-opaque switching in Candida albicans, an opportunistic human fungal pathogen, and is associated with its pathogenicity and commensality. Wor1 contains a conserved DNA-binding region at the N-terminus, consisting of two conserved segments (WOPRa and WOPRb) connected by a non-conserved linker that can bind to specific DNA sequences of the promoter regions and then regulates the transcription. Here, we report the crystal structure of the C. albicans Wor1 WOPR segments in complex with a double-stranded DNA corresponding to one promoter region of WOR1. The sequentially separated WOPRa and WOPRb are structurally interwound together to form a compact globular domain that we term the WOPR domain. The WOPR domain represents a new conserved fungal-specific DNA-binding domain which uses primarily a conserved loop to recognize and interact specifically with a conserved 6-bp motif of the DNA in both minor and major grooves. The protein-DNA interactions are essential for WOR1 transcriptional regulation and white-to-opaque switching. The structural and biological data together reveal the molecular basis for the recognition and binding specificity of the WOPR domain with its specific DNA sequences and the function of Wor1 in the activation of transcription.
PDB ID: 4QTJDownload
MMDB ID: 122397
PDB Deposition Date: 2014/7/8
Updated in MMDB: 2017/12
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Candida albicans SC5314
Similar Structures:
Biological Unit for 4QTJ: trimeric; determined by software (PISA)
Molecular Components in 4QTJ
Label Count Molecule
Protein (1 molecule)
White-opaque Regulator 1(Gene symbol: WOR1)
Molecule annotation
Nucleotides(2 molecules)
DNA (5'-d(*ap*ap*ap*ap*gp*tp*tp*tp*ap*ap*cp*tp*t)-3')
Molecule annotation
DNA (5'-d(*ap*ap*gp*tp*tp*ap*ap*ap*cp*tp*tp*tp*t)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB