4QR9: Crystal Structure Of Two Hmgb1 Box A Domains Cooperating To Underwind And Kink A Dna

Citation:
Abstract
High-mobility group protein 1 (HMGB1) is an essential and ubiquitous DNA architectural factor that influences a myriad of cellular processes. HMGB1 contains two DNA-binding domains, box A and box B, which have little sequence specificity but have remarkable abilities to underwind and bend DNA. Although HMGB1 box A is thought to be responsible for the majority of HMGB1-DNA interactions with pre-bent or kinked DNA, little is known about how it recognizes unmodified DNA. Here, the crystal structure of HMGB1 box A bound to an AT-rich DNA fragment is reported at a resolution of 2 A. Two box A domains of HMGB1 collaborate in an unusual configuration in which the Phe37 residues of both domains stack together and intercalate the same CG base pair, generating highly kinked DNA. This represents a novel mode of DNA recognition for HMGB proteins and reveals a mechanism by which structure-specific HMG boxes kink linear DNA.
PDB ID: 4QR9Download
MMDB ID: 130459
PDB Deposition Date: 2014/6/30
Updated in MMDB: 2015/07
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4QR9: hexameric; determined by author
Molecular Components in 4QR9
Label Count Molecule
Proteins (2 molecules)
2
High Mobility Group Protein B1(Gene symbol: Hmgb1)
Molecule annotation
Nucleotide(1 molecule)
4
DNA (5'-d(*ap*tp*ap*tp*cp*gp*ap*tp*ap*t)-3')
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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