4QPQ: Mechanistic Basis Of Plasmid-specific Dna Binding Of The F Plasmid Regulatory Protein, Tram

Citation:
Abstract
The conjugative transfer of bacterial F plasmids relies on TraM, a plasmid-encoded protein that recognizes multiple DNA sites to recruit the plasmid to the conjugative pore. In spite of the high degree of amino acid sequence conservation between TraM proteins, many of these proteins have markedly different DNA binding specificities that ensure the selective recruitment of a plasmid to its cognate pore. Here we present the structure of F TraM RHH (ribbon-helix-helix) domain bound to its sbmA site. The structure indicates that a pair of TraM tetramers cooperatively binds an underwound sbmA site containing 12 base pairs per turn. The sbmA is composed of 4 copies of a 5-base-pair motif, each of which is recognized by an RHH domain. The structure reveals that a single conservative amino acid difference in the RHH beta-ribbon between F and pED208 TraM changes its specificity for its cognate 5-base-pair sequence motif. Specificity is also dictated by the positioning of 2-base-pair spacer elements within sbmA; in F sbmA, the spacers are positioned between motifs 1 and 2 and between motifs 3 and 4, whereas in pED208 sbmA, there is a single spacer between motifs 2 and 3. We also demonstrate that a pair of F TraM tetramers can cooperatively bind its sbmC site with an affinity similar to that of sbmA in spite of a lack of sequence similarity between these DNA elements. These results provide a basis for the prediction of the DNA binding properties of the family of TraM proteins.
PDB ID: 4QPQDownload
MMDB ID: 123450
PDB Deposition Date: 2014/6/24
Updated in MMDB: 2014/12
Experimental Method:
x-ray diffraction
Resolution: 3.11  Å
Source Organism:
Escherichia coli K-12
Similar Structures:
Biological Unit for 4QPQ: decameric; determined by author and by software (PISA)
Molecular Components in 4QPQ
Label Count Molecule
Proteins (8 molecules)
8
Relaxosome Protein Tram(Gene symbol: traM)
Molecule annotation
Nucleotides(2 molecules)
1
Sbma Dna1
Molecule annotation
1
Sbma Dna2
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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