4QH0: Crystal Structure Of Nuca From Streptococcus Agalactiae With Magnesium Ion Bound

Citation:
Abstract
The group B pathogen Streptococcus agalactiae commonly populates the human gut and urogenital tract, and is a major cause of infection-based mortality in neonatal infants and in elderly or immunocompromised adults. Nuclease A (GBS_NucA), a secreted DNA/RNA nuclease, serves as a virulence factor for S. agalactiae, facilitating bacterial evasion of the human innate immune response. GBS_NucA efficiently degrades the DNA matrix component of neutrophil extracellular traps (NETs), which attempt to kill and clear invading bacteria during the early stages of infection. In order to better understand the mechanisms of DNA substrate binding and catalysis of GBS_NucA, the high-resolution structure of a catalytically inactive mutant (H148G) was solved by X-ray crystallography. Several mutants on the surface of GBS_NucA which might influence DNA substrate binding and catalysis were generated and evaluated using an imidazole chemical rescue technique. While several of these mutants severely inhibited nuclease activity, two mutants (K146R and Q183A) exhibited significantly increased activity. These structural and biochemical studies have greatly increased our understanding of the mechanism of action of GBS_NucA in bacterial virulence and may serve as a foundation for the structure-based drug design of antibacterial compounds targeted to S. agalactiae.
PDB ID: 4QH0Download
MMDB ID: 124870
PDB Deposition Date: 2014/5/26
Updated in MMDB: 2014/11
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Similar Structures:
Biological Unit for 4QH0: monomeric; determined by author
Molecular Components in 4QH0
Label Count Molecule
Protein (1 molecule)
1
DNA-entry Nuclease (Competence-specific Nuclease)
Molecule annotation
Chemicals (11 molecules)
1
1
2
8
3
2
* Click molecule labels to explore molecular sequence information.

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