4Q8H: Structure Of The Saccharomyces Cerevisiae Pan2 Pseudoubiquitin- Hydrolase-rnase Module

Pan2-Pan3 is a conserved complex involved in the shortening of mRNA poly(A) tails, the initial step in eukaryotic mRNA turnover. We show that recombinant Saccharomyces cerevisiae Pan2-Pan3 can deadenylate RNAs in vitro without needing the poly(A)-binding protein Pab1. The crystal structure of an active ~200-kDa core complex reveals that Pan2 and Pan3 interact with an unusual 1:2 stoichiometry imparted by the asymmetric nature of the Pan3 homodimer. An extended region of Pan2 wraps around Pan3 and provides a major anchoring point for complex assembly. A Pan2 module formed by the pseudoubiquitin-hydrolase and RNase domains latches onto the Pan3 pseudokinase with intertwined interactions that orient the deadenylase active site toward the A-binding site of the interacting Pan3. The molecular architecture of Pan2-Pan3 suggests how the nuclease and its pseudokinase regulator act in synergy to promote deadenylation.
PDB ID: 4Q8HDownload
MMDB ID: 120532
PDB Deposition Date: 2014/4/27
Updated in MMDB: 2014/07
Experimental Method:
x-ray diffraction
Resolution: 3.1  Å
Source Organism:
Similar Structures:
Biological Unit for 4Q8H: monomeric; determined by author
Molecular Components in 4Q8H
Label Count Molecule
Protein (1 molecule)
Pab-dependent Poly(a)-specific Ribonuclease Subunit Pan2(Gene symbol: PAN2)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB