4Q76: Crystal structure of Nfs2 C384S mutant, the plastidial cysteine desulfurase from Arabidopsis thaliana

The chloroplastic Arabidopsis thaliana Nfs2 (AtNfs2) is a group II pyridoxal 5'-phosphate-dependent cysteine desulfurase that is involved in the initial steps of iron-sulfur cluster biogenesis. The group II cysteine desulfurases require the presence of sulfurtransferases such as SufE proteins for optimal activity. Compared with group I cysteine desulfurases, proteins of this group contains a smaller extended lobe harbouring the catalytic cysteine and have a beta-hairpin constraining the active site. Here, two crystal structures of AtNfs2 are reported: a wild-type form with the catalytic cysteine in a persulfide-intermediate state and a C384S variant mimicking the resting state of the enzyme. In both structures the well conserved Lys241 covalently binds pyridoxal 5'-phosphate, forming an internal aldimine. Based on available homologous bacterial complexes, a model of a complex between AtNfs2 and the SufE domain of its biological partner AtSufE1 is proposed, revealing the nature of the binding sites.
PDB ID: 4Q76Download
MMDB ID: 123064
PDB Deposition Date: 2014/4/24
Updated in MMDB: 2017/12
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 4Q76: dimeric; determined by author and by software (PISA)
Molecular Components in 4Q76
Label Count Molecule
Proteins (2 molecules)
Cysteine Desulfurase 2, Chloroplastic(Gene symbol: CPNIFS)
Molecule annotation
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