4Q3E: Pyld Cocrystallized With L-ornithine-nd-d-lysine And Nad+

Citation:
Abstract
The dehydrogenase PylD catalyzes the ultimate step of the pyrrolysine pathway by converting the isopeptide L-lysine-Nepsilon-3R-methyl-D-ornithine to the 22nd proteinogenic amino acid. In this study, we demonstrate how PylD can be harnessed to oxidize various isopeptides to novel amino acids by combining chemical synthesis with enzyme kinetics and X-ray crystallography. The data enable a detailed description of the PylD reaction trajectory for the biosynthesis of pyrroline and tetrahydropyridine rings as constituents of pyrrolysine analogues.
PDB ID: 4Q3EDownload
MMDB ID: 119975
PDB Deposition Date: 2014/4/11
Updated in MMDB: 2014/08
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 4Q3E: monomeric; determined by author
Molecular Components in 4Q3E
Label Count Molecule
Protein (1 molecule)
1
Pyld, Pyrrolysine Synthase
Molecule annotation
Chemicals (6 molecules)
1
1
2
1
3
1
4
1
5
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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