4PZI: Zinc finger region of MLL2 in complex with CpG DNA

Citation:
Abstract
The CXXC domain, first identified as the reader of unmodified CpG dinucleotide, plays important roles in epigenetic regulation by targeting various activities to CpG islands. Here we systematically measured and compared the DNA-binding selectivities of all known human CXXC domains by different binding assays, and complemented the existing function-based classification of human CXXC domains with a classification based on their DNA selectivities. Through a series of crystal structures of CXXC domains with DNA ligands, we unravel the molecular mechanisms of how these CXXC domains, including single CXXC domains and tandem CXXC-PHD domains, recognize distinct DNA ligands, which further supports our classification of human CXXC domains and also provides insights into selective recruitment of chromatin modifiers to their respective targets via CXXC domains recognizing different genomic DNA sequences. Our study facilitates the understanding of the relationship between the DNA-binding specificities of the CXXC proteins and their biological functions.
PDB ID: 4PZIDownload
MMDB ID: 120527
PDB Deposition Date: 2014/3/31
Updated in MMDB: 2014/06
Experimental Method:
x-ray diffraction
Resolution: 2.15  Å
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 4PZI: trimeric; determined by software (PISA)
Molecular Components in 4PZI
Label Count Molecule
Protein (1 molecule)
1
Histone-lysine N-methyltransferase 2B(Gene symbol: KMT2B)
Molecule annotation
Nucleotide(1 molecule)
2
DNA (5'-d(*gp*cp*cp*ap*cp*cp*gp*gp*tp*gp*gp*c)-3')
Molecule annotation
Chemicals (3 molecules)
1
2
2
1
Molecule information is not avaliable.
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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