4PN8: A De Novo Designed Pentameric Coiled Coil Cc-pent

Citation:
Abstract
The design of protein sequences that fold into prescribed de novo structures is challenging. General solutions to this problem require geometric descriptions of protein folds and methods to fit sequences to these. The alpha-helical coiled coils present a promising class of protein for this and offer considerable scope for exploring hitherto unseen structures. For alpha-helical barrels, which have more than four helices and accessible central channels, many of the possible structures remain unobserved. Here, we combine geometrical considerations, knowledge-based scoring, and atomistic modeling to facilitate the design of new channel-containing alpha-helical barrels. X-ray crystal structures of the resulting designs match predicted in silico models. Furthermore, the observed channels are chemically defined and have diameters related to oligomer state, which present routes to design protein function.
PDB ID: 4PN8Download
MMDB ID: 124062
PDB Deposition Date: 2014/5/23
Updated in MMDB: 2014/12
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Similar Structures:
Biological Unit for 4PN8: pentameric; determined by author and by software (PISA)
Molecular Components in 4PN8
Label Count Molecule
Proteins (5 molecules)
5
Cc-pent
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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