4PMV: Crystal structure of a novel reducing-end xylose-releasing exo-oligoxylanase (XynA) belonging to GH10 family (space group P43212)

Citation:
Abstract
Xanthomonas pathogens attack a variety of economically relevant plants, and their xylan CUT system (carbohydrate utilization with TonB-dependent outer membrane transporter system) contains two major xylanase-related genes, xynA and xynB, which influence biofilm formation and virulence by molecular mechanisms that are still elusive. Herein, we demonstrated that XynA is a rare reducing end xylose-releasing exo-oligoxylanase and not an endo-beta-1,4-xylanase as predicted. Structural analysis revealed that an insertion in the beta7-alpha7 loop induces dimerization and promotes a physical barrier at the +2 subsite conferring this unique mode of action within the GH10 family. A single mutation that impaired dimerization became XynA active against xylan, and high endolytic activity was achieved when this loop was tailored to match a canonical sequence of endo-beta-1,4-xylanases, supporting our mechanistic model. On the other hand, the divergent XynB proved to be a classical endo-beta-1,4-xylanase, despite the low sequence similarity to characterized GH10 xylanases. Interestingly, this enzyme contains a calcium ion bound nearby to the glycone-binding region, which is required for catalytic activity and structural stability. These results shed light on the molecular basis for xylan degradation by Xanthomonas and suggest how these enzymes synergistically assist infection and pathogenesis. Our findings indicate that XynB contributes to breach the plant cell wall barrier, providing nutrients and facilitating the translocation of effector molecules, whereas the exo-oligoxylanase XynA possibly participates in the suppression of oligosaccharide-induced immune responses.
PDB ID: 4PMVDownload
MMDB ID: 123632
PDB Deposition Date: 2014/5/22
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 3.001  Å
Source Organism:
Similar Structures:
Biological Unit for 4PMV: dimeric; determined by author and by software (PISA)
Molecular Components in 4PMV
Label Count Molecule
Proteins (2 molecules)
2
Endo-1,4-beta-xylanase a
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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