4PIT: Crystal Structure of Banana Lectin H84T bound to dimannose

Citation:
Abstract
A key effector route of the Sugar Code involves lectins that exert crucial regulatory controls by targeting distinct cellular glycans. We demonstrate that a single amino-acid substitution in a banana lectin, replacing histidine 84 with a threonine, significantly reduces its mitogenicity, while preserving its broad-spectrum antiviral potency. X-ray crystallography, NMR spectroscopy, and glycocluster assays reveal that loss of mitogenicity is strongly correlated with loss of pi-pi stacking between aromatic amino acids H84 and Y83, which removes a wall separating two carbohydrate binding sites, thus diminishing multivalent interactions. On the other hand, monovalent interactions and antiviral activity are preserved by retaining other wild-type conformational features and possibly through unique contacts involving the T84 side chain. Through such fine-tuning, target selection and downstream effects of a lectin can be modulated so as to knock down one activity, while preserving another, thus providing tools for therapeutics and for understanding the Sugar Code.
PDB ID: 4PITDownload
MMDB ID: 133826
PDB Deposition Date: 2014/5/9
Updated in MMDB: 2017/12
Experimental Method:
x-ray diffraction
Resolution: 1.55  Å
Source Organism:
Similar Structures:
Biological Unit for 4PIT: dimeric; determined by author and by software (PISA)
Molecular Components in 4PIT
Label Count Molecule
Proteins (2 molecules)
2
Ripening-associated Protein
Molecule annotation
Chemicals (9 molecules)
1
7
2
2
* Click molecule labels to explore molecular sequence information.

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