4PD3: Crystal Structure of Rigor-Like Human Nonmuscle Myosin-2B

Citation:
Abstract
We determined the crystal structure of the motor domain of human non-muscle myosin 2B (NM-2B) in a nucleotide-free state and at a resolution of 2.8 A. The structure shows the motor domain with an open active site and the large cleft that divides the 50 kDa domain in a closed state. Compared to other rigor-like myosin motor domain structures, our structure shows subtle but significant conformational changes in regions important for actin binding and mechanochemical coupling. Moreover, our crystal structure helps to rationalize the impact of myosin, heavy chain 9 (MYH9)-related disease mutations Arg709Cys and Arg709His on the kinetic and functional properties of NM-2B and of the closely related non-muscle myosin 2A (NM-2A).
PDB ID: 4PD3Download
MMDB ID: 124651
PDB Deposition Date: 2014/4/17
Updated in MMDB: 2014/12
Experimental Method:
x-ray diffraction
Resolution: 2.84  Å
Similar Structures:
Biological Unit for 4PD3: monomeric; determined by author and by software (PISA)
Molecular Components in 4PD3
Label Count Molecule
Protein (1 molecule)
1
Nonmuscle Myosin Heavy Chain B, Alpha-actinin a Chimera Protein
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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