4OJD: Crystal Structure Of A C-terminally Truncated Trimeric Ectodomain Of The C. Elegans Fusion Protein Eff-1 G260a/d321e/d322e Mutant

Citation:
Abstract
Cell-cell fusion proteins are essential in development. Here we show that the C. elegans cell-cell fusion protein EFF-1 is structurally homologous to viral class II fusion proteins. The 2.6 A crystal structure of the EFF-1 trimer displays the same 3D fold and quaternary conformation of postfusion class II viral fusion proteins, although it lacks a nonpolar "fusion loop," indicating that it does not insert into the target membrane. EFF-1 was previously shown to be required in both cells for fusion, and we show that blocking EFF-1 trimerization blocks the fusion reaction. Together, these data suggest that whereas membrane fusion driven by viral proteins entails leveraging of a nonpolar loop, EFF-1-driven fusion of cells entails trans-trimerization such that transmembrane segments anchored in the two opposing membranes are brought into contact at the tip of the EFF-1 trimer to then, analogous to SNARE-mediated vesicle fusion, zip the two membranes into one.
PDB ID: 4OJDDownload
MMDB ID: 119448
PDB Deposition Date: 2014/1/21
Updated in MMDB: 2014/05
Experimental Method:
x-ray diffraction
Resolution: 2.26  Å
Source Organism:
Similar Structures:
Biological Unit for 4OJD: trimeric; determined by author and by software (PISA)
Molecular Components in 4OJD
Label Count Molecule
Proteins (3 molecules)
3
Eff-1a
Molecule annotation
Chemicals (15 molecules)
1
12
2
3
* Click molecule labels to explore molecular sequence information.

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