4OGE: Crystal Structure Of The Type Ii-c Cas9 Enzyme From Actinomyces Naeslundii

Citation:
Abstract
Type II CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems use an RNA-guided DNA endonuclease, Cas9, to generate double-strand breaks in invasive DNA during an adaptive bacterial immune response. Cas9 has been harnessed as a powerful tool for genome editing and gene regulation in many eukaryotic organisms. We report 2.6 and 2.2 angstrom resolution crystal structures of two major Cas9 enzyme subtypes, revealing the structural core shared by all Cas9 family members. The architectures of Cas9 enzymes define nucleic acid binding clefts, and single-particle electron microscopy reconstructions show that the two structural lobes harboring these clefts undergo guide RNA-induced reorientation to form a central channel where DNA substrates are bound. The observation that extensive structural rearrangements occur before target DNA duplex binding implicates guide RNA loading as a key step in Cas9 activation.
PDB ID: 4OGEDownload
MMDB ID: 117549
PDB Deposition Date: 2014/1/15
Updated in MMDB: 2014/03
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 4OGE: monomeric; determined by author and by software (PISA)
Molecular Components in 4OGE
Label Count Molecule
Protein (1 molecule)
1
HNH Endonuclease Domain Protein
Molecule annotation
Chemicals (6 molecules)
1
1
2
3
3
2
* Click molecule labels to explore molecular sequence information.

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