National Center for
4O9M: Human Dna Polymerase Beta Complexed With Adenylated Tetrahydrofuran (abasic Site) Containing Dna
Role of polymerase beta in complementing aprataxin deficiency during abasic-site base excision repair
Nat. Struct. Mol. Biol. (2014) 21 p.497-499
DNA polymerase beta (pol beta) lyase removal of 5'-deoxyribose phosphate (5'-dRP) from base excision repair (BER) intermediates is critical in mammalian BER involving the abasic site. We found that pol beta also removes 5'-adenylated dRP from BER intermediates after abortive ligation. The crystal structure of a human pol beta-DNA complex showed the 5'-AMP-dRP group positioned in the lyase active site. Pol beta expression rescued methyl methanesulfonate sensitivity in aprataxin (hnt3)- and FEN1 (rad27)-deficient yeast.