4O9M: Human Dna Polymerase Beta Complexed With Adenylated Tetrahydrofuran (abasic Site) Containing Dna

Citation:
Abstract
DNA polymerase beta (pol beta) lyase removal of 5'-deoxyribose phosphate (5'-dRP) from base excision repair (BER) intermediates is critical in mammalian BER involving the abasic site. We found that pol beta also removes 5'-adenylated dRP from BER intermediates after abortive ligation. The crystal structure of a human pol beta-DNA complex showed the 5'-AMP-dRP group positioned in the lyase active site. Pol beta expression rescued methyl methanesulfonate sensitivity in aprataxin (hnt3)- and FEN1 (rad27)-deficient yeast.
PDB ID: 4O9MDownload
MMDB ID: 119776
PDB Deposition Date: 2014/1/2
Updated in MMDB: 2014/06
Experimental Method:
x-ray diffraction
Resolution: 2.29  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4O9M: tetrameric; determined by author and by software (PISA)
Molecular Components in 4O9M
Label Count Molecule
Protein (1 molecule)
1
DNA Polymerase Beta(Gene symbol: POLB)
Molecule annotation
Nucleotides(3 molecules)
1
DNA (5'-d(*cp*cp*gp*ap*cp*ap*gp*cp*gp*cp*ap*tp*cp*ap*gp*c)- 3')
Molecule annotation
1
DNA (5'-d(*gp*cp*tp*gp*ap*tp*gp*cp*gp*cp*c)-3')
Molecule annotation
1
(5'-d(p*gp*tp*cp*gp*g)-3')
Molecule annotation
Chemicals (3 molecules)
1
1
2
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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