4NII: Crystal Structure Of Alkb D135i Mutant Protein With Cofactors Bound To Dsdna Containing M6a/a

Citation:
Abstract
The AlkB family demethylases AlkB, FTO, and ALKBH5 recognize differentially methylated RNA/DNA substrates, which results in their distinct biological roles. Here we identify key active-site residues that contribute to their substrate specificity. Swapping such active-site residues between the demethylases leads to partially switched demethylation activities. Combined evidence from X-ray structures and enzyme kinetics suggests a role of the active-site residues in substrate recognition. Such a divergent active-site sequence may aid the design of selective inhibitors that can discriminate these homologue RNA/DNA demethylases.
PDB ID: 4NIIDownload
MMDB ID: 119220
PDB Deposition Date: 2013/11/6
Updated in MMDB: 2016/06
Experimental Method:
x-ray diffraction
Resolution: 1.62  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4NII: trimeric; determined by author and by software (PISA)
Molecular Components in 4NII
Label Count Molecule
Protein (1 molecule)
1
Alpha-ketoglutarate-dependent Dioxygenase Alkb(Gene symbol: alkB)
Molecule annotation
Nucleotides(2 molecules)
1
5'-d(*tp*ap*gp*gp*tp*ap*ap*(6ma)p*ap*cp*cp*gp*t)-3'
Molecule annotation
1
5'-d(*ap*ap*cp*gp*gp*tp*ap*tp*tp*ap*cp*cp*t)-3'
Molecule annotation
Chemicals (3 molecules)
1
1
2
1
3
1
Molecule information is not avaliable.
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Citing MMDB
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