4NDM: Structure of the AB18.1 TCR

The nature of the antigens recognized by gammadelta T cells and their potential recognition of major histocompatibility complex (MHC)-like molecules has remained unclear. Members of the CD1 family of lipid-presenting molecules are suggested ligands for Vdelta1 TCR-expressing gammadelta T cells, the major gammadelta lymphocyte population in epithelial tissues. We crystallized a Vdelta1 TCR in complex with CD1d and the self-lipid sulfatide, revealing the unusual recognition of CD1d by germline Vdelta1 residues spanning all complementarity-determining region (CDR) loops, as well as sulfatide recognition separately encoded by nongermline CDR3delta residues. Binding and functional analysis showed that CD1d presenting self-lipids, including sulfatide, was widely recognized by gut Vdelta1+ gammadelta T cells. These findings provide structural demonstration of MHC-like recognition of a self-lipid by gammadelta T cells and reveal the prevalence of lipid recognition by innate-like T cell populations.
PDB ID: 4NDMDownload
MMDB ID: 116111
PDB Deposition Date: 2013/10/27
Updated in MMDB: 2013/12
Experimental Method:
x-ray diffraction
Resolution: 3.0065  Å
Source Organism:
Similar Structures:
Biological Unit for 4NDM: dimeric; determined by author and by software (PISA)
Molecular Components in 4NDM
Label Count Molecule
Proteins (2 molecules)
T-cell Gamma Protein,t-cell Receptor Beta-2 Chain C Region
Molecule annotation
Human NKT TCR Alpha Chain
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB