4N4A: Cystal structure of Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

The 5' cap of human messenger RNA contains 2'-O-methylation of the first and often second transcribed nucleotide that is important for its processing, translation and stability. Human enzymes that methylate these nucleotides, termed CMTr1 and CMTr2, respectively, have recently been identified. However, the structures of these enzymes and their mechanisms of action remain unknown. In the present study, we solve the crystal structures of the active CMTr1 catalytic domain in complex with a methyl group donor and a capped oligoribonucleotide, thereby revealing the mechanism of specific recognition of capped RNA. This mechanism differs significantly from viral enzymes, thus providing a framework for their specific targeting. Based on the crystal structure of CMTr1, a comparative model of the CMTr2 catalytic domain is generated. This model, together with mutational analysis, leads to the identification of residues involved in RNA and methyl group donor binding.
PDB ID: 4N4ADownload
MMDB ID: 116867
PDB Deposition Date: 2013/10/8
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.35  Å
Source Organism:
Similar Structures:
Biological Unit for 4N4A: monomeric; determined by author
Molecular Components in 4N4A
Label Count Molecule
Protein (1 molecule)
Cap-specific mRNA (Nucleoside-2'-o-)-methyltransferase 1(Gene symbol: CMTR1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB