4MZP: Mazf From S. Aureus Crystal Form Iii, C2221, 2.7 A

The Staphylococcus aureus genome contains three toxin-antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-type SaMazF toxin. The protein is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperatures. Crystallographic and nuclear magnetic resonance (NMR) solution studies show a well-defined dimer. Differences in structure and dynamics between the X-ray and NMR structural ensembles are found in three loop regions, two of which undergo motions that are of functional relevance. The same segments also show functionally relevant dynamics in the distantly related CcdB family despite divergence of function. NMR chemical shift mapping and analysis of residue conservation in the MazF family suggests a conserved mode for the inhibition of MazF by MazE.
PDB ID: 4MZPDownload
MMDB ID: 120095
PDB Deposition Date: 2013/9/30
Updated in MMDB: 2014/06
Experimental Method:
x-ray diffraction
Resolution: 2.7  Å
Source Organism:
Similar Structures:
Biological Unit for 4MZP: dimeric; determined by author and by software (PISA)
Molecular Components in 4MZP
Label Count Molecule
Proteins (2 molecules)
Mazf mRNA Interferase
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB