4MZ0: Structure Of A Ketosynthase-acyltransferase Di-domain From Module Curl Of The Curacin A Polyketide Synthase

Citation:
Abstract
Modular type I polyketide synthases (PKSs) are versatile biosynthetic systems that initiate, successively elongate, and modify acyl chains. Intermediate transfer between modules is mediated via docking domains, which are attractive targets for PKS pathway engineering to produce natural product analogs. We identified a class 2 docking domain in cyanobacterial PKSs and determined crystal structures for two docking domain pairs, revealing a distinct class 2 docking strategy for promoting intermediate transfer. The selectivity of class 2 docking interactions, demonstrated in binding and biochemical assays, could be altered by mutagenesis. We determined the ideal fusion location for exchanging class 1 and class 2 docking domains and demonstrated effective polyketide chain transfer in heterologous modules. Thus, class 2 docking domains are tools for rational bioengineering of a broad range of PKSs containing either class 1 or 2 docking domains.
PDB ID: 4MZ0Download
MMDB ID: 117076
PDB Deposition Date: 2013/9/28
Updated in MMDB: 2014/02
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Similar Structures:
Biological Unit for 4MZ0: dimeric; determined by author and by software (PISA)
Molecular Components in 4MZ0
Label Count Molecule
Proteins (2 molecules)
2
Curl
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

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