4MMU: Crystal Structure Of Prefusion-stabilized Rsv F Variant Ds-cav1 At Ph 5.5

Respiratory syncytial virus (RSV) is the leading cause of hospitalization for children under 5 years of age. We sought to engineer a viral antigen that provides greater protection than currently available vaccines and focused on antigenic site O, a metastable site specific to the prefusion state of the RSV fusion (F) glycoprotein, as this site is targeted by extremely potent RSV-neutralizing antibodies. Structure-based design yielded stabilized versions of RSV F that maintained antigenic site O when exposed to extremes of pH, osmolality, and temperature. Six RSV F crystal structures provided atomic-level data on how introduced cysteine residues and filled hydrophobic cavities improved stability. Immunization with site O-stabilized variants of RSV F in mice and macaques elicited levels of RSV-specific neutralizing activity many times the protective threshold.
PDB ID: 4MMUDownload
MMDB ID: 115322
PDB Deposition Date: 2013/9/9
Updated in MMDB: 2013/11
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Human respiratory syncytial virus A2
Similar Structures:
Biological Unit for 4MMU: hexameric; determined by author and by software (PISA)
Molecular Components in 4MMU
Label Count Molecule
Proteins (6 molecules)
Fusion Glycoprotein F2
Molecule annotation
Fusion Glycoprotein F1 Fused With Fibritin Trimerization Domain(Gene symbol: wac)
Molecule annotation
Chemicals (18 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB