4MMU: Crystal Structure Of Prefusion-stabilized Rsv F Variant Ds-cav1 At Ph 5.5

Citation:
Abstract
Respiratory syncytial virus (RSV) is the leading cause of hospitalization for children under 5 years of age. We sought to engineer a viral antigen that provides greater protection than currently available vaccines and focused on antigenic site O, a metastable site specific to the prefusion state of the RSV fusion (F) glycoprotein, as this site is targeted by extremely potent RSV-neutralizing antibodies. Structure-based design yielded stabilized versions of RSV F that maintained antigenic site O when exposed to extremes of pH, osmolality, and temperature. Six RSV F crystal structures provided atomic-level data on how introduced cysteine residues and filled hydrophobic cavities improved stability. Immunization with site O-stabilized variants of RSV F in mice and macaques elicited levels of RSV-specific neutralizing activity many times the protective threshold.
PDB ID: 4MMUDownload
MMDB ID: 115322
PDB Deposition Date: 2013/9/9
Updated in MMDB: 2013/11
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Human respiratory syncytial virus A2
Similar Structures:
Biological Unit for 4MMU: hexameric; determined by author and by software (PISA)
Molecular Components in 4MMU
Label Count Molecule
Proteins (6 molecules)
3
Fusion Glycoprotein F2
Molecule annotation
3
Fusion Glycoprotein F1 Fused With Fibritin Trimerization Domain(Gene symbol: wac)
Molecule annotation
Chemicals (18 molecules)
1
12
2
3
3
3
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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