4MH1: Crystal Structure And Functional Studies Of Quinoprotein L-sorbose Dehydrogenase From Ketogulonicigenium Vulgare Y25

The crystal structure of the L-sorbose dehydrogenase (SDH) from Ketogulonicigenium vulgare Y25 has been determined at 2.7 A resolution using the molecular replacement method. The overall structure of SDH is similar to that of other quinoprotein dehydrogenases; consisting of an eight bladed beta-propeller PQQ domain and protrusion loops. We identified a stable homodimer in crystal and demonstrated its existence in solution by sedimentation velocity measurement. By biochemical characterization of the SDH in vitro, using L-sorbose as substrate and cytochrome c551 as electron acceptor, we revealed cytochrome c551 acting as physiological primary electron acceptor for SDH.
PDB ID: 4MH1Download
MMDB ID: 121697
PDB Deposition Date: 2013/8/29
Updated in MMDB: 2014/07
Experimental Method:
x-ray diffraction
Resolution: 2.7  Å
Source Organism:
Similar Structures:
Biological Unit for 4MH1: tetrameric; determined by author and by software (PISA)
Molecular Components in 4MH1
Label Count Molecule
Proteins (4 molecules)
Sorbose Dehydrogenase
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

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