4M3X: Rb69 Dna Polymerase Ternary Complex With Dt/dg At Position N-5 Of Primer/template Duplex

Citation:
Abstract
Recent structures of DNA polymerase complexes with dGMPCPP/dT and dCTP/dA mispairs at the insertion site have shown that they adopt Watson-Crick geometry in the presence of Mn(2+) indicating that the tautomeric or ionization state of the base has changed. To see whether the tautomeric or ionization state of base-pair could be affected by its microenvironment, we determined 10 structures of an RB69 DNA polymerase quadruple mutant with dG/dT or dT/dG mispairs at position n-1 to n-5 of the Primer/Template duplex. Different shapes of the mispairs, including Watson-Crick geometry, have been observed, strongly suggesting that the local environment of base-pairs plays an important role in their tautomeric or ionization states.
PDB ID: 4M3XDownload
MMDB ID: 117500
PDB Deposition Date: 2013/8/6
Updated in MMDB: 2014/04
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4M3X: trimeric; determined by author and by software (PISA)
Molecular Components in 4M3X
Label Count Molecule
Protein (1 molecule)
1
DNA Polymerase(Gene symbol: 43)
Molecule annotation
Nucleotides(2 molecules)
1
DNA Template
Molecule annotation
1
DNA Primer
Molecule annotation
Chemicals (6 molecules)
1
1
2
5
* Click molecule labels to explore molecular sequence information.

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