4LSV: Crystal Structure Of Broadly And Potently Neutralizing Antibody 3bnc117 In Complex With Hiv-1 Clade C C1086 Gp120

Citation:
Abstract
Antibodies of the VRC01 class neutralize HIV-1, arise in diverse HIV-1-infected donors, and are potential templates for an effective HIV-1 vaccine. However, the stochastic processes that generate repertoires in each individual of >10(12) antibodies make elicitation of specific antibodies uncertain. Here we determine the ontogeny of the VRC01 class by crystallography and next-generation sequencing. Despite antibody-sequence differences exceeding 50%, antibody-gp120 cocrystal structures reveal VRC01-class recognition to be remarkably similar. B cell transcripts indicate that VRC01-class antibodies require few specific genetic elements, suggesting that naive-B cells with VRC01-class features are generated regularly by recombination. Virtually all of these fail to mature, however, with only a few-likely one-ancestor B cell expanding to form a VRC01-class lineage in each donor. Developmental similarities in multiple donors thus reveal the generation of VRC01-class antibodies to be reproducible in principle, thereby providing a framework for attempts to elicit similar antibodies in the general population.
PDB ID: 4LSVDownload
MMDB ID: 112896
PDB Deposition Date: 2013/7/23
Updated in MMDB: 2013/09
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Human immunodeficiency virus 1
Similar Structures:
Biological Unit for 4LSV: trimeric; determined by author and by software (PISA)
Molecular Components in 4LSV
Label Count Molecule
Proteins (3 molecules)
1
Envelope Glycoprotein Gp120
Molecule annotation
1
Heavy Chain of Antibody 3bnc117
Molecule annotation
1
Light Chain of Antibody 3bnc117
Molecule annotation
Chemicals (12 molecules)
1
11
2
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.