4LSR: Crystal Structure Of Broadly And Potently Neutralizing Antibody Vrc- Ch31 In Complex With Hiv-1 Clade A/e Stran 93th057 Gp120 With Loop D And Loop V5 From Clade A Strain Ker_2018_11

Antibodies of the VRC01 class neutralize HIV-1, arise in diverse HIV-1-infected donors, and are potential templates for an effective HIV-1 vaccine. However, the stochastic processes that generate repertoires in each individual of >10(12) antibodies make elicitation of specific antibodies uncertain. Here we determine the ontogeny of the VRC01 class by crystallography and next-generation sequencing. Despite antibody-sequence differences exceeding 50%, antibody-gp120 cocrystal structures reveal VRC01-class recognition to be remarkably similar. B cell transcripts indicate that VRC01-class antibodies require few specific genetic elements, suggesting that naive-B cells with VRC01-class features are generated regularly by recombination. Virtually all of these fail to mature, however, with only a few-likely one-ancestor B cell expanding to form a VRC01-class lineage in each donor. Developmental similarities in multiple donors thus reveal the generation of VRC01-class antibodies to be reproducible in principle, thereby providing a framework for attempts to elicit similar antibodies in the general population.
PDB ID: 4LSRDownload
MMDB ID: 112892
PDB Deposition Date: 2013/7/23
Updated in MMDB: 2013/09
Experimental Method:
x-ray diffraction
Resolution: 2.28  Å
Source Organism:
Human immunodeficiency virus 1
Similar Structures:
Biological Unit for 4LSR: trimeric; determined by author
Molecular Components in 4LSR
Label Count Molecule
Proteins (3 molecules)
Envelope Glycoprotein Gp120 With Loop D and V5 From Strain Ker_2018_11
Molecule annotation
Heavy Chain of Antibody Vrc-ch31
Molecule annotation
Light Chain of Antibody Vrc-ch31 (N70d Mutation)
Molecule annotation
Chemicals (14 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB