4LMJ: Glic Liganded-closed-channel Conformation, Mutant T25'a

Citation:
Abstract
Cryoelectron microscopy and X-ray crystallography have recently been used to generate structural models that likely represent the unliganded closed-channel conformation and the fully liganded open-channel conformation of different members of the nicotinic-receptor superfamily. To characterize the structure of the closed-channel conformation in its liganded state, we identified a number of positions in the loop between transmembrane segments 2 (M2) and 3 (M3) of a proton-gated ortholog from the bacterium Gloeobacter violaceus (GLIC) where mutations to alanine reduce the liganded-gating equilibrium constant, and solved the crystal structures of two such mutants (T25'A and Y27'A) at pH approximately 4.0. At the level of backbone atoms, the liganded closed-channel model presented here differs from the liganded open-channel structure of GLIC in the pre-M1 linker, the M3-M4 loop, and much more prominently, in the extracellular half of the pore lining, where the more pronounced tilt of the closed-channel M2 alpha-helices toward the pore's long axis narrows the permeation pathway. On the other hand, no differences between the liganded closed-channel and open-channel models could be detected at the level of the extracellular domain, where conformational changes are expected to underlie the low-to-high proton-affinity switch that drives gating of proton-bound channels. Thus, the liganded closed-channel model is nearly indistinguishable from the recently described "locally closed" structure. However, because cross-linking strategies (which could have stabilized unstable conformations) and mutations involving ionizable side chains (which could have affected proton-gated channel activation) were purposely avoided, we favor the notion that this structure represents one of the end states of liganded gating rather than an unstable intermediate.
PDB ID: 4LMJDownload
MMDB ID: 114756
PDB Deposition Date: 2013/7/10
Updated in MMDB: 2013/11
Experimental Method:
x-ray diffraction
Resolution: 3.44  Å
Source Organism:
Similar Structures:
Biological Unit for 4LMJ: pentameric; determined by author and by software (PISA)
Molecular Components in 4LMJ
Label Count Molecule
Proteins (5 molecules)
5
Proton-gated Ion Channel(Gene symbol: glr4197)
Molecule annotation
Chemicals (11 molecules)
1
5
2
1
3
5
* Click molecule labels to explore molecular sequence information.

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