4LI0: Crystal Structure Of Gdp-bound Rab8:grab

Small G-proteins of the Ras superfamily control the temporal and spatial coordination of intracellular signaling networks by acting as molecular on/off switches. Guanine nucleotide exchange factors (GEFs) regulate the activation of these G-proteins through catalytic replacement of GDP by GTP. During nucleotide exchange, three distinct substrate.enzyme complexes occur: a ternary complex with GDP at the start of the reaction (G-protein.GEF.GDP), an intermediary nucleotide-free binary complex (G-protein.GEF), and a ternary GTP complex after productive G-protein activation (G-protein.GEF.GTP). Here, we show structural snapshots of the full nucleotide exchange reaction sequence together with the G-protein substrates and products using Rabin8/GRAB (GEF) and Rab8 (G-protein) as a model system. Together with a thorough enzymatic characterization, our data provide a detailed view into the mechanism of Rabin8/GRAB-mediated nucleotide exchange.
PDB ID: 4LI0Download
MMDB ID: 114268
PDB Deposition Date: 2013/7/1
Updated in MMDB: 2013/12
Experimental Method:
x-ray diffraction
Resolution: 3.3  Å
Source Organism:
Similar Structures:
Biological Unit for 4LI0: trimeric; determined by author and by software (PISA)
Molecular Components in 4LI0
Label Count Molecule
Proteins (3 molecules)
Ras-related Protein Rab-8a(Gene symbol: RAB8A)
Molecule annotation
Guanine Nucleotide Exchange Factor for Rab-3a(Gene symbol: RAB3IL1)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB