4LGN: The Structure Of Acidothermus Cellulolyticus Family 74 Glycoside Hydrolase

Citation:
Abstract
Here, a 1.82 A resolution X-ray structure of a glycoside hydrolase family 74 (GH74) enzyme from Acidothermus cellulolyticus is reported. The resulting structure was refined to an R factor of 0.150 and an Rfree of 0.196. Structural analysis shows that five related structures have been reported with a secondary-structure similarity of between 75 and 89%. The five similar structures were all either Clostridium thermocellum or Geotrichum sp. M128 GH74 xyloglucanases. Structural analysis indicates that the A. cellulolyticus GH74 enzyme is an endoxyloglucanase, as it lacks a characteristic loop that blocks one end of the active site in exoxyloglucanases. Superimposition with the C. thermocellum GH74 shows that Asp451 and Asp38 are the catalytic residues.
PDB ID: 4LGNDownload
MMDB ID: 115866
PDB Deposition Date: 2013/6/28
Updated in MMDB: 2013/12
Experimental Method:
x-ray diffraction
Resolution: 1.82  Å
Source Organism:
Similar Structures:
Biological Unit for 4LGN: monomeric; determined by author and by software (PISA)
Molecular Components in 4LGN
Label Count Molecule
Protein (1 molecule)
1
Cellulose-binding, Family II
Molecule annotation
Chemicals (36 molecules)
1
3
2
16
3
4
4
3
5
6
6
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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