4L74: Ca2+-bound MthK RCK domain at 1.9 Angstrom with single ligand

Ligand binding sites within proteins can interact by allosteric mechanisms to modulate binding affinities and control protein function. Here we present crystal structures of the regulator of K+ conductance (RCK) domain from a K+ channel, MthK, which reveal the structural basis of allosteric coupling between two Ca2+ regulatory sites within the domain. Comparison of RCK domain crystal structures in a range of conformations and with different numbers of regulatory Ca2+ ions bound, combined with complementary electrophysiological analysis of channel gating, suggests chemical interactions that are important for modulation of ligand binding and subsequent channel opening.
PDB ID: 4L74Download
MMDB ID: 114522
PDB Deposition Date: 2013/6/13
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 1.841  Å
Source Organism:
Similar Structures:
Biological Unit for 4L74: dimeric; determined by author and by software (PISA)
Molecular Components in 4L74
Label Count Molecule
Proteins (2 molecules)
Calcium-gated Potassium Channel Mthk(Gene symbol: MTH_RS07260)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB