4L3C: Structure Of Hla-a2 In Complex With D76n B2m Mutant And Ny-eso1 Double Mutant

To form extracellular aggregates, amyloidogenic proteins bypass the intracellular quality control, which normally targets unfolded/aggregated polypeptides. Human D76N beta2-microglobulin (beta2m) variant is the prototype of unstable and amyloidogenic protein that forms abundant extracellular fibrillar deposits. Here we focus on the role of the class I major histocompatibility complex (MHCI) in the intracellular stabilization of D76N beta2m. Using biophysical and structural approaches, we show that the MHCI containing D76N beta2m (MHCI76) displays stability, dissociation patterns, and crystal structure comparable with those of the MHCI with wild type beta2m. Conversely, limited proteolysis experiments show a reduced protease susceptibility for D76N beta2m within the MHCI76 as compared with the free variant, suggesting that the MHCI has a chaperone-like activity in preventing D76N beta2m degradation within the cell. Accordingly, D76N beta2m is normally assembled in the MHCI and circulates as free plasma species in a transgenic mouse model.
PDB ID: 4L3CDownload
MMDB ID: 116258
PDB Deposition Date: 2013/6/5
Updated in MMDB: 2013/12
Experimental Method:
x-ray diffraction
Resolution: 2.64  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4L3C: trimeric; determined by author and by software (PISA)
Molecular Components in 4L3C
Label Count Molecule
Proteins (3 molecules)
HLA Class I Histocompatibility Antigen, A-2 Alpha Chain(Gene symbol: HLA-A)
Molecule annotation
Beta-2-microglobulin(Gene symbol: B2M)
Molecule annotation
Ny-eso1 Double Mutant (1y, 9V)
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB