4KJ5: Control Of Ribosomal Subunit Rotation By Elongation Factor G

Citation:
Abstract
Protein synthesis by the ribosome requires the translocation of transfer RNAs and messenger RNA by one codon after each peptide bond is formed, a reaction that requires ribosomal subunit rotation and is catalyzed by the guanosine triphosphatase (GTPase) elongation factor G (EF-G). We determined 3 angstrom resolution x-ray crystal structures of EF-G complexed with a nonhydrolyzable guanosine 5'-triphosphate (GTP) analog and bound to the Escherichia coli ribosome in different states of ribosomal subunit rotation. The structures reveal that EF-G binding to the ribosome stabilizes switch regions in the GTPase active site, resulting in a compact EF-G conformation that favors an intermediate state of ribosomal subunit rotation. These structures suggest that EF-G controls the translocation reaction by cycles of conformational rigidity and relaxation before and after GTP hydrolysis.
MMDB ID: 111941
PDB Deposition Date: 2013/5/3
Updated in MMDB: 2013/08
Experimental Method:
x-ray diffraction
Resolution: 2.9  Å
Source Organism:
Similar Structures:
Merged PDB IDs:4KJ5 4KJ6 4KJ7 4KJ8 4KJ9 4KJA 4KJB 4KJC
Asymmetric Unit for 4KJ5: 217-meric
Molecular Components in 4KJ5
Label Count Molecule
Proteins (202 molecules)
4
50S Ribosomal Protein L2
Molecule annotation
4
50S Ribosomal Protein L3
Molecule annotation
4
50S Ribosomal Protein L4
Molecule annotation
4
50S Ribosomal Protein L5
Molecule annotation
4
50S Ribosomal Protein L6
Molecule annotation
4
50S Ribosomal Protein L9
Molecule annotation
4
50S Ribosomal Protein L11
Molecule annotation
4
50S Ribosomal Protein L13
Molecule annotation
4
50S Ribosomal Protein L14
Molecule annotation
4
50S Ribosomal Protein L15
Molecule annotation
4
50S Ribosomal Protein L16
Molecule annotation
4
50S Ribosomal Protein L17
Molecule annotation
4
50S Ribosomal Protein L18
Molecule annotation
4
50S Ribosomal Protein L19
Molecule annotation
4
50S Ribosomal Protein L20
Molecule annotation
4
50S Ribosomal Protein L21
Molecule annotation
4
50S Ribosomal Protein L22
Molecule annotation
4
50S Ribosomal Protein L23
Molecule annotation
4
50S Ribosomal Protein L24
Molecule annotation
4
50S Ribosomal Protein L25
Molecule annotation
4
50S Ribosomal Protein L27
Molecule annotation
4
50S Ribosomal Protein L28
Molecule annotation
4
50S Ribosomal Protein L29
Molecule annotation
4
50S Ribosomal Protein L30
Molecule annotation
4
50S Ribosomal Protein L32
Molecule annotation
4
50S Ribosomal Protein L33
Molecule annotation
4
50S Ribosomal Protein L34
Molecule annotation
4
50S Ribosomal Protein L35
Molecule annotation
4
50S Ribosomal Protein L36
Molecule annotation
2
50S Ribosomal Protein L10
Molecule annotation
4
30S Ribosomal Protein S2
Molecule annotation
4
30S Ribosomal Protein S3
Molecule annotation
4
30S Ribosomal Protein S4
Molecule annotation
4
30S Ribosomal Protein S5
Molecule annotation
4
30S Ribosomal Protein S6
Molecule annotation
4
30S Ribosomal Protein S7
Molecule annotation
4
30S Ribosomal Protein S8
Molecule annotation
4
30S Ribosomal Protein S9
Molecule annotation
4
30S Ribosomal Protein S10
Molecule annotation
4
30S Ribosomal Protein S11
Molecule annotation
4
30S Ribosomal Protein S12
Molecule annotation
4
30S Ribosomal Protein S13
Molecule annotation
4
30S Ribosomal Protein S14
Molecule annotation
4
30S Ribosomal Protein S15
Molecule annotation
4
30S Ribosomal Protein S16
Molecule annotation
4
30S Ribosomal Protein S17
Molecule annotation
4
30S Ribosomal Protein S18
Molecule annotation
4
30S Ribosomal Protein S19
Molecule annotation
4
30S Ribosomal Protein S20
Molecule annotation
4
30S Ribosomal Protein S21
Molecule annotation
4
Elongation Factor G
Molecule annotation
Nucleotides(4 molecules)
4
23S rRNA
Molecule annotation
4
5S rRNA
Molecule annotation
4
16S rRNA
Molecule annotation
3
Viomycin
Molecule annotation
Chemicals (723 molecules)
1
715
2
4
3
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.