4JQ5: Crystal structure of the human Nup49CCS2+3* coiled-coil segment

The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. We present the reconstitution and interdisciplinary analyses of the ~425-kilodalton inner ring complex (IRC), which forms the central transport channel and diffusion barrier of the NPC, revealing its interaction network and equimolar stoichiometry. The Nsp1*Nup49*Nup57 channel nucleoporin heterotrimer (CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96. The CNT*Nic96 structure reveals that Nic96 functions as an assembly sensor that recognizes the three-dimensional architecture of the CNT, thereby mediating the incorporation of a defined CNT state into the NPC. We propose that the IRC adopts a relatively rigid scaffold that recruits the CNT to primarily form the diffusion barrier of the NPC, rather than enabling channel dilation.
PDB ID: 4JQ5Download
MMDB ID: 123340
PDB Deposition Date: 2013/3/20
Updated in MMDB: 2014/10
Experimental Method:
x-ray diffraction
Resolution: 2.195  Å
Source Organism:
Similar Structures:
Biological Unit for 4JQ5: tetrameric; determined by author
Molecular Components in 4JQ5
Label Count Molecule
Proteins (4 molecules)
Nucleoporin P58/p45(Gene symbol: NUP58)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB