4JK9: Open And Closed Forms Of Wild-type Human Prp8 Rnase H-like Domain With Bound Co Ion

Splicing of pre-mRNAs in eukaryotes is catalyzed by the spliceosome, a large RNA-protein metalloenzyme. The catalytic center of the spliceosome involves a structure comprising the U2 and U6 snRNAs and includes a metal bound by U6 snRNA. The precise architecture of the splicesome active site, however, and the question of whether it includes protein components, remains unresolved. A wealth of evidence places the protein PRP8 at the heart of the spliceosome through assembly and catalysis. Here we provide evidence that the RNase H domain of PRP8 undergoes a conformational switch between the two steps of splicing, rationalizing yeast prp8 alleles that promote either the first or second step. We also show that this switch unmasks a metal-binding site involved in the second step. Together, these data establish that PRP8 is a metalloprotein that promotes exon ligation within the spliceosome.
PDB ID: 4JK9Download
MMDB ID: 110316
PDB Deposition Date: 2013/3/9
Updated in MMDB: 2013/06
Experimental Method:
x-ray diffraction
Resolution: 1.5  Å
Source Organism:
Similar Structures:
Biological Unit for 4JK9: monomeric; determined by author and by software (PISA)
Molecular Components in 4JK9
Label Count Molecule
Protein (1 molecule)
Pre-mrna-processing-splicing Factor 8(Gene symbol: PRPF8)
Molecule annotation
Chemicals (5 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB