4JI4: Crystal Structure Of 30s Ribosomal Subunit From Thermus Thermophilus

Citation:
Abstract
The ribosome decodes mRNA by monitoring the geometry of codon-anticodon base-pairing using a set of universally conserved 16S rRNA nucleotides within the conformationally dynamic decoding site. By applying single-molecule FRET and X-ray crystallography, we have determined that conditional-lethal, streptomycin-dependence mutations in ribosomal protein S12 interfere with tRNA selection by allowing conformational distortions of the decoding site that impair GTPase activation of EF-Tu during the tRNA selection process. Distortions in the decoding site are reversed by streptomycin or by a second-site suppressor mutation in 16S rRNA. These observations encourage a refinement of the current model for decoding, wherein ribosomal protein S12 and the decoding site collaborate to optimize codon recognition and substrate discrimination during the early stages of the tRNA selection process.
PDB ID: 4JI4Download
MMDB ID: 114880
PDB Deposition Date: 2013/3/5
Updated in MMDB: 2013/12
Experimental Method:
x-ray diffraction
Resolution: 3.69  Å
Source Organism:
Similar Structures:
Biological Unit for 4JI4: 21-meric; determined by author
Molecular Components in 4JI4
Label Count Molecule
Proteins (20 molecules)
1
Ribosomal Protein S2(Gene symbol: rpsB)
Molecule annotation
1
Ribosomal Protein S3(Gene symbol: rpsC)
Molecule annotation
1
Ribosomal Protein S4(Gene symbol: rpsD)
Molecule annotation
1
Ribosomal Protein S5(Gene symbol: rpsE)
Molecule annotation
1
Ribosomal Protein S6(Gene symbol: rpsF)
Molecule annotation
1
Ribosomal Protein S7(Gene symbol: TTHA1696)
Molecule annotation
1
Ribosomal Protein S8
Molecule annotation
1
Ribosomal Protein S9(Gene symbol: rpsI)
Molecule annotation
1
Ribosomal Protein S10(Gene symbol: rpsJ)
Molecule annotation
1
Ribosomal Protein S11(Gene symbol: TTHA1666)
Molecule annotation
1
Ribosomal Protein S12
Molecule annotation
1
Ribosomal Protein S13(Gene symbol: rpsM)
Molecule annotation
1
Ribosomal Protein S14
Molecule annotation
1
Ribosomal Protein S15(Gene symbol: rpsO)
Molecule annotation
1
Ribosomal Protein S16(Gene symbol: rpsP)
Molecule annotation
1
Ribosomal Protein S17
Molecule annotation
1
Ribosomal Protein S18(Gene symbol: rpsR)
Molecule annotation
1
Ribosomal Protein S19(Gene symbol: rpsS)
Molecule annotation
1
Ribosomal Protein S20(Gene symbol: rpsT)
Molecule annotation
1
Ribosomal Protein THX(Gene symbol: TTHA1396)
Molecule annotation
Nucleotide(1 molecule)
1
16S rRNA
Molecule annotation
Chemicals (276 molecules)
1
274
2
2
* Click molecule labels to explore molecular sequence information.

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