4IT7: Crystal Structure Of Al-cpi

Citation:
Abstract
Immunosuppression associated with infections of nematode parasites has been documented. Cysteine protease inhibitor (CPI) released by the nematode parasites is identified as one of the major modulators of host immune response. In this report, we demonstrated that the recombinant CPI protein of Ascaris lumbricoides (Al-CPI) strongly inhibited the activities of cathepsin L, C, S, and showed weaker effect to cathepsin B. Crystal structure of Al-CPI was determined to 2.1 A resolution. Two segments of Al-CPI, loop 1 and loop 2, were proposed as the key structure motifs responsible for Al-CPI binding with proteases and its inhibitory activity. Mutations at loop 1 and loop 2 abrogated the protease inhibition activity to various extents. These results provide the molecular insight into the interaction between the nematode parasite and its host and will facilitate the development of anthelmintic agents or design of anti-autoimmune disease drugs.
PDB ID: 4IT7Download
MMDB ID: 116993
PDB Deposition Date: 2013/1/17
Updated in MMDB: 2014/06
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 4IT7: monomeric; determined by author and by software (PISA)
Molecular Components in 4IT7
Label Count Molecule
Protein (1 molecule)
1
CPI
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.