4HQC: Crystal Structure Of A Green-to-red Photoconvertible Dronpa, Pcdronpa In The Red-on-state

Citation:
Abstract
Advanced imaging techniques crucially depend on the labels used. In this work, we present the structure-guided design of a fluorescent protein that displays both reversibly photochromic and green-to-red photoconversion behavior. We first designed ffDronpa, a mutant of the photochromic fluorescent protein Dronpa that matures up to three times faster while retaining its interesting photochromic features. Using a combined evolutionary and structure-driven rational design strategy, we developed a green-to-red photoconvertible ffDronpa mutant, called pcDronpa, and explored different optimization strategies that resulted in its improved version, pcDronpa2. This fluorescent probe combines a high brightness with low photobleaching and photoblinking. We herein show that, despite its tetrameric nature, pcDronpa2 allows for multimodal subdiffraction imaging by sequentially imaging a given sample using both super-resolution fluctuation imaging and localization microscopy.
PDB ID: 4HQCDownload
MMDB ID: 115361
PDB Deposition Date: 2012/10/25
Updated in MMDB: 2013/11
Experimental Method:
x-ray diffraction
Resolution: 2.05  Å
Source Organism:
Similar Structures:
Biological Unit for 4HQC: tetrameric; determined by author and by software (PISA)
Molecular Components in 4HQC
Label Count Molecule
Proteins (4 molecules)
4
Fluorescent Protein Dronpa
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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