4H9G: Probing EF-Tu with a very small brominated fragment library identifies the CCA pocket

Citation:
Abstract
High-quality crystals of Thermus thermophilus EF-Tu in the GTP-bound conformation at 1.7-2.7 A resolution were used to test 18 small organic molecules, all brominated for confident identification in the anomalous difference maps. From this relatively small collection, it was possible to identify a small molecule bound in the functionally important tRNA CCA-end binding pocket. The antibiotic GE2270 A is known to interact with the same pocket in EF-Tu and to disrupt the association with tRNA. Bromide could be located from peaks in the anomalous map in data truncated to very low resolution without refining the structure. Considering the speed with which diffraction data can be collected today, it is proposed that it is worthwhile to collect the extra data from fragment screens while crystals are at hand to increase the knowledge of biological function and drug binding in an experimental structural context.
PDB ID: 4H9GDownload
MMDB ID: 113380
PDB Deposition Date: 2012/9/24
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 1.93  Å
Source Organism:
Similar Structures:
Biological Unit for 4H9G: monomeric; determined by author and by software (PISA)
Molecular Components in 4H9G
Label Count Molecule
Protein (1 molecule)
1
Elongation Factor Tu-a
Molecule annotation
Chemicals (12 molecules)
1
1
2
1
3
1
4
8
5
1
* Click molecule labels to explore molecular sequence information.

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