4GZK: Structure and Interactions of the RNA-dependent RNA Polymerase From Bacteriophage Phi12

Citation:
Abstract
We have determined the structure of P2, the self-priming RdRp from cystovirus phi12 in two crystal forms (A, B) at resolutions of 1.7 A and 2.1 A. Form A contains Mg(2+) bound at a site that deviates from the canonical noncatalytic position seen in form B. These structures provide insight into the temperature sensitivity of a ts-mutant. However, the tunnel through which template ssRNA accesses the active site is partially occluded by a flexible loop; this feature, along with suboptimal positioning of other structural elements that prevent the formation of a stable initiation complex, indicate an inactive conformation in crystallo.
PDB ID: 4GZKDownload
MMDB ID: 112027
PDB Deposition Date: 2012/9/6
Updated in MMDB: 2013/09
Experimental Method:
x-ray diffraction
Resolution: 1.69  Å
Source Organism:
Similar Structures:
Biological Unit for 4GZK: monomeric; determined by author and by software (PISA)
Molecular Components in 4GZK
Label Count Molecule
Protein (1 molecule)
1
RNA-dependent RNA Polymerase P2
Molecule annotation
Chemicals (6 molecules)
1
5
2
1
* Click molecule labels to explore molecular sequence information.

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