4GK5: Crystal Structure Of Human Rev3-rev7-rev1-polkappa Complex

In addition to DNA repair pathways, cells utilize translesion DNA synthesis (TLS) to bypass DNA lesions during replication. During TLS, Y-family DNA polymerase (Poleta, Polkappa, Poli and Rev1) inserts specific nucleotide opposite preferred DNA lesions, and then Polzeta consisting of two subunits, Rev3 and Rev7, carries out primer extension. Here, we report the complex structures of Rev3-Rev7-Rev1(CTD) and Rev3-Rev7-Rev1(CTD)-Polkappa(RIR). These two structures demonstrate that Rev1(CTD) contains separate binding sites for Polkappa and Rev7. Our BIAcore experiments provide additional support for the notion that the interaction between Rev3 and Rev7 increases the affinity of Rev7 and Rev1. We also verified through FRET experiment that Rev1, Rev3, Rev7 and Polkappa form a stable quaternary complex in vivo, thereby suggesting an efficient switching mechanism where the "inserter" polymerase can be immediately replaced by an "extender" polymerase within the same quaternary complex.
PDB ID: 4GK5Download
MMDB ID: 108298
PDB Deposition Date: 2012/8/10
Updated in MMDB: 2013/03
Experimental Method:
x-ray diffraction
Resolution: 3.21  Å
Source Organism:
Similar Structures:
Biological Unit for 4GK5: tetrameric; determined by author and by software (PISA)
Molecular Components in 4GK5
Label Count Molecule
Proteins (4 molecules)
Mitotic Spindle Assembly Checkpoint Protein Mad2b(Gene symbol: MAD2L2)
Molecule annotation
DNA Polymerase Zeta Catalytic Subunit(Gene symbol: REV3L)
Molecule annotation
DNA Repair Protein Rev1(Gene symbol: REV1)
Molecule annotation
DNA Polymerase Kappa(Gene symbol: POLK)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB