4GBR: N-terminal T4 Lysozyme Fusion Facilitates Crystallization Of A G Protein Coupled Receptor

A highly crystallizable T4 lysozyme (T4L) was fused to the N-terminus of the beta(2) adrenergic receptor (beta(2)AR), a G-protein coupled receptor (GPCR) for catecholamines. We demonstrate that the N-terminal fused T4L is sufficiently rigid relative to the receptor to facilitate crystallogenesis without thermostabilizing mutations or the use of a stabilizing antibody, G protein, or protein fused to the 3rd intracellular loop. This approach adds to the protein engineering strategies that enable crystallographic studies of GPCRs alone or in complex with a signaling partner.
PDB ID: 4GBRDownload
MMDB ID: 104268
PDB Deposition Date: 2012/7/27
Updated in MMDB: 2012/10
Experimental Method:
x-ray diffraction
Resolution: 3.99  Å
Source Organism:
Escherichia virus T4
Similar Structures:
Biological Unit for 4GBR: dimeric; determined by author
Molecular Components in 4GBR
Label Count Molecule
Proteins (2 molecules)
Beta-2 Adrenergic Receptor(Gene symbol: ADRB2)
Molecule annotation
Lysozyme(Gene symbol: e)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB