4FUP: Structural Basis For Zn2+-dependent Intercellular Adhesion In Staphylococcal Biofilms

Significance Under adverse environmental conditions, bacteria can form specialized antibiotic-resistant colonies called "biofilms." In Staphylococcus epidermidis biofilms, a protein, Aap, links bacterial cells together but does so only in the presence of zinc ions. We have determined the atomic structure of an adhesive portion of Aap bound to zinc. The protein adopts an elongated, flexible fold with zinc ions bridging two protein chains. The mode of assembly indicates that Aap is likely to form twisted rope-like structures between bacterial cells. These data provide clues about regions of the protein that could be targeted to prevent intercellular adhesion in the developing biofilm.
PDB ID: 4FUPDownload
MMDB ID: 106721
PDB Deposition Date: 2012/6/28
Updated in MMDB: 2013/02
Experimental Method:
x-ray diffraction
Resolution: 2.51  Å
Source Organism:
Similar Structures:
Biological Unit for 4FUP: dimeric; determined by author and by software (PISA)
Molecular Components in 4FUP
Label Count Molecule
Proteins (2 molecules)
Accumulation Associated Protein
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB